Fluoroquinolones for Infectious Diseases Treatment

Fluoroquinolones for Infectious Diseases Treatment 1.10 Pharmaceuticals 1.10.2 Fluoroquinolones Fluoroquinolones are extensively used for treatment of various infectious diseases. [[1]]. Because of their extensive Gram negative treatment, quinolone antibiotics were initially used for the treatment urinary tract related diseases. Higher drug concentrations promote their effectiveness in the treatment of urinary infections. lomefloxacin, levofloxacin ciprofloxacin, ofloxacin, enrofloxacin, and gatifloxacin have higher renal clearance to analyze the activity of the kidney and urine concentration test measures the ability of the kidneys to excrete water. Fluoroquinolones enter the environment by different routes including municipal and industrial wastewater effluent. The recent studies show that from many parts of the world reported the presence of fluoroquinolones in surface water bodies at concentrations ranging from non-detectable to around 50 ng dm-3 [[2]]. The existence and addition of fluoroquinolone antibiotics in aquatic environments, at very low concentrations, may cause affect to the ecosystem and human health. They require development of the different oxidation methods for the transformation of fluoroquinolones in water during water treatment. Disinfection processes (e.g., chlorination, oxidation, and UV irradiation) appear to result in considerable addition of fluoroquinolone and their transformation during municipal treatment of waste before to release into water stream [[3]]. An added disinfectant may undergo transformation reactions with antibacterial agents during water treatment. Sodium hypochlorite is a commonly intended for chlorination of water during disinfection process, and also potassium permanganate may be also used for disinfection processes[[4][5]]. Considering the occurrence of chlorine in municipal wastewater and drinking water disinfection processes, reactions with aqueous chlorine species likely play a particularly important role in the environmental fate of fluoroquinolones. Levofloxacin Levofloxacin is in a class of antibacterial agent called fluoroquinolones. It is used for the treatment of certain bacterial infections. Levofloxacin is used to treat certain infections such as urinary tract, chronic pneumonia, bronchitis, kidney and skin infections. Levofloxacin may used to prevent anthrax in people who may have been open to anthrax germs in the air. It works by destroying bacteria that causes infections. Antibiotics will not work for viral infections, flu, colds, or other diseases. Lomefloxacin Structure of Lomefloxacin (LMF) Lomefloxacin is also class of fluoroquinolones antibacterial agent.This used to treat a wide range of bacterial infections. It is used to treat bacterial infections including bronchitis and urinary tract infections. Lomefloxacin is associated with photo toxicity and central nervous system adverse effects [[6]]. 1.10.3 Oxazolidinones The oxazolidinones, a new class of synthetic antibacterial agents have a distinctive mechanism of to contorol bacterial protein synthesis. The oxazolidinone to be approved for clinical use, show in-vitro activity against many important resistant harmful organisms. Clinical trials verified the action in the setting of pneumonia soft-tissue, and skin infections, and infections due to vancomycin-resistant. [[7]]. Linezolid Linezolid is an antibacterial agent used to treat certain serious bacterial infections that have not taken action from other antibacterial agents. Linezolid is not only used to treats bacterial infections, but also for viral infections. Unnecessary use or over use of any antibiotic can lead to its reduces effectiveness. Linezolid is a relatively safe drug; it can be used in patients of all ages and in people with poor kidney function or liver disease [[8]]. The current study was undertaken to elucidate reaction products, kinetics, and mechanism between free available chlorine (FAC) or permanganate with fluoroquinolones class of antibacterial agents such as levofloxacin (LFC) Lomefloxacin (LMF) and oxazolidinone class of antibacterial agent linezolid (LNZ). Which are the most popular for disease control and prevention in the recently are used. 1.10.1. Routes of pharmaceuticals entering into the environment The figures 1.4.a. and 1.4.b. and figure 1.5 shows that a large fraction of clinically prescribed antibiotic dose is discharged into municipal waste water systems due to incomplete metabolism of antibiotics within the human body. (Rain) runoff water carries the hospital wastes to rivers and contaminates the river water. In recent years, there has been growing concern about the presence of pharmaceuticals in the aquatic environment. Continuous exposure of antibiotics to bacterial communities, promotes the bacteria to develop antibiotic resistance. Possible induction of antibiotic resistance in bacteria is directly related to human health. The action of antibiotics during water treatment process clearly plays a significant role in this regard. Antibacterials and other pharmaceutical are having the tendency to persist contaminants in the water supply is of increasing concern in the field of environmental toxicology. Several national and international bodies have reported the presence of antibacterials in surface water, ground water, drinking water, and waste water [[9]]. Antibacterials were primarily observed as â€Å"wonder medicines† mainly because they were introduced from surgical drains or spontaneous cure were available to treat serious bacterial diseases. Many classes of these antibacterial agents were discovered in the last five to six decades. These include penicillins, sulfonamides, trimethoprim, chloramphenicol, cephalosporins, colimycins, tetracyclines, lincosamides etc, [[10]]. Understanding the fate and transportation of antibacterial agents in the aquatic environment is vital to properly assess the risk associated with these emerging contaminants [[11]]. What happens to these antibacterial agents during municipal water treatment? Municipal water treatment essentially involve following processes; The steps involved in conventional water treatment method are shown in the above flow diagram. The aeration process is carried out to remove the odor from the water. The filtration is the removal of the solids, specially suspended matter, by passing the water through a granular media (sand, coal, diatomaceous earth, granular activated carbon). The colloidal particles pass through the filtration process and removed using coagulants in the flocculation process. The micro contaminants, which are dissolved in water, can easily pass through aeration, filtration and flocculation processes but they may react with the disinfectants in the last process. What are the commonly used disinfectants? Chlorine: Chlorine gas, NaOCl, Bleaching powder, Conventional water treatment Chloramines: Weak disinfectant and low rate of reaction Ozone: Costly UV/H2O2: Costly Not suitable for Municipal water treatment KMnO4: Potassium permanganate is usually applied for waste water treatment 1.11 Disinfection Disinfection is the process of killing pathogenic organisms like bacteria and viruses in the drinking water supply. It is the last step in the treatment and is necessary to supply a â€Å"bacteriologically free† drinking water for the general public usage. Disinfection is the necessary step before the public water supplies. Chlorination is the treatment technique of killing harmful microorganisms in water supplies. 1.11.1 Chlorination An added layer of complexity in this problem lies in the potential bio-transformation antibacterial agents can undergo during drinking water chlorination. Chlorination, in the form of sodium hypochlorite, is a common mechanism of drinking water disinfection. [[12]]. Chlorination has been shown degradation of certain parent drugs in drinking water [[13]]. The effect of chlorination has been studied for several non antibacterials. However, these studies are few in comparison to the variety of pharmaceutical contaminants our environment faces [[14]]. A long-term objective of this research work is to know the fate of antibacterial in the water supply when they are exposed to chlorination and oxidation in the drinking water treatment process. Microorganisms can be found in raw water like rivers, lakes and groundwater. Some microorganisms may cause diseases in human and are called pathogens. These pathogens existing in water can be transmitted through a drinking water distribution system, causes water related diseases. The use of chlorine in the water treatment process was originally directed to the primary function of disinfection. Chlorination is one of the methods that can be used to make germ-free water. This method was first used over a hundred years ago, and is still it is continued. It is a chemical disinfection method that uses various types of chlorine or chlorine-containing substances for the oxidation and disinfection of what will be the potable water source. 1.11.2 Importance and benefits of chlorination of water Many investigations and studies have been carried out to make sure success in new treatment plants using chlorine as a cleaning agent. An important benefit of chlorination is that it has effective against viruses and bacteria. The three most common types of chlorinating agents used in water treatment are: Ca (OCl)2 (calcium hypochlorite), NaOCl (sodium hypochlorite), and Cl2 (chlorine) gas, Any type of chlorinating agent is added to water during the water treatment process will lead to form of hypochlorous acid (HOCl) and hypochlorite ion (OCl), which are the main disinfecting species. Of the two disinfecting species, hypochlorous acid is the most effective. The amount of each compound present in the water is dependent on the pH level of the water. At lower pH levels, the hypochlorous acid will dominant. The quantity of chlorine that is required to disinfect water is depends on the impurities in the water. The amount of chlorine that is required to satisfy all the impurities is termed the ‘chlorine demand. Once the chlorine demand has been reached is called breakpoint chlorination i.e., the addition of chlorine to water until the chlorine demand has been fulfilled. After the breakpoint, any extra chlorine added will result in free chlorine residual, residual chlorine can react with a number of different contaminants present in raw water The main purpose of chlorination is to disinfect water, but it also has many other benefits. Unlike some of the other disinfection methods like ozonation and ultraviolet radiation, chlorination is able to provide a residual to reduce the chance of growing pathogens in water storage tanks or the water distribution system. 1.11.3 Types of chlorinating agents 1.11.3 .1 Chlorine Gas Chlorine gas is good disinfectant, but it is toxic to more than just waterborne pathogens; it is also toxic to humans. When chlorine gas (Cl2) is added to the water (H2O), it hydrolyzes rapidly to produce hypochlorous acid (HOCl) and the hypochlorous acid will then dissociate into hypochlorite ions (OCl) and hydrogen ions (H+). Because hydrogen ions are produced, the water will become more acidic (the pH of the water will decrease). The amount of dissociation depends on the original pH of the water. If the pH of the water is below a 6.5, nearly no dissociation will occur and the hypochlorous acid will dominate. A pH above 8.5 will see a complete dissociation of chlorine, and hypochlorite ions will dominate. A pH between 6.5 and 8.5 will see both hypochlorous acid and hypochlorite ions present in the water. Together, the hypochlorous acid and the hypochlorite ions are referred to as free chlorine. Hypchlorous acid is the more effective disinfectant, and therefore, a lower pH is preferred for disinfection. 1.11.3.2 Calcium hypochlorite Calcium hypochlorite Ca (OCl) 2 is made up of the calcium salts of hypochlorous acid. When treating water, a lesser amount of calcium hypochlorite is needed than if using chlorine gas. When calcium hypochlorite is added to water, hypochlorite and calcium ions are produced. Instead of decreasing the pH like chlorine gas does, calcium hypochlorite increases the pH of the water. However, hypochlorous acid and hypochlorite concentrations are still dependent on the pH of the water; therefore by decreasing the pH of the water, hypochlorous acid will still be present in the water. As a result, calcium hypochlorite and chlorine gas both produce the same type of residuals. 1.11.3 .3 Sodium hypochlorite Sodium hypochlorite (NaOCl) is made up of the sodium salts of hypochlorous acid and is a chlorine-containing compound that can be used as a disinfectant. It is produced when chlorine gas is dissolved into a sodium hydroxide solution. It is in liquid form, clear with a light yellow color, and has a strong chlorine smell. Sodium hypochlorite is extremely corrosive and must be stored in a cool, dark, and dry place. Sodium hypochlorite will naturally decompose; therefore it cannot be stored for more than one month at a time. Of all the different types of chlorine available for use, this is the easiest to handle. Like calcium hypochlorite, sodium hypochlorite will also produce a hypochlorite ion, but instead of calcium ions, sodium ions are produced. NaOCl will also increase the pH of the water through the formation of hypochlorite ions. To obtain hypochlorous acid, which is a more effective disinfectant, the pH of the water should be decreased. In drinking water, the concentration of chlorine is usually very low and is thus not a concern in acute exposure. More of a concern is the long term risk of cancer due to chronic exposure to chlorinated water. Chlorination is a very conventional method of water disinfection that has been used from several years. It is efficient for destroying viruses and bacteria. 1.12 Aqueous chlorination chemistry In water treatment, gaseous chlorine Cl2 or hypochlorite are commonly used for chlorination processes. Chlorine gas (Cl2) hydrolyzes in water according to the following reaction: Fig.1.5. Relative distribution of main aqueous chlorine species as a function of pH at 25 ËÅ ¡C and for a chloride concentration Where k1 and k-1 values, calculated at  µ=0 M and 25ËÅ ¡C from Wang and Margerum, are 22.3 s-1 and 4.3Ãâ€"104 M-2 s-1, respectively. For temperatures between 0 and 25 1ËÅ ¡C, KCl2 ranges from 1.3Ãâ€"10-4 to 5.1Ãâ€"10-4 [[15]]. Hypochlorous acid resulting from reaction (1), is a weak acid which dissociates in aqueous solution: With KHOCl reported in literature between 1.5Ãâ€"10-8 (pKaHOCl,0ËÅ ¡C = 7.82) and 2.9Ãâ€"10-8 (pKaHOCl,25ËÅ ¡C= 7.54) for temperatures between 0 and 25 ËÅ ¡C [[16]]. Under typical water treatment conditions in the pH range 6–9, hypochlorous acid and hypochlorite are the main chlorine species. Depending on the temperature and pH level, different distributions of aqueous chlorine species are observed. Fig. 1.6. Shows the distribution of HOCl and ClO as a function of the pH at 25ËÅ ¡C and for a chloride concentration of 5Ãâ€"10-3 M (177.5mgL-1). For these high chloride concentrations, Fig. 1 6. shows that Cl2 hydrolysis is almost complete at pH >4. Therefore, Cl2 can usually be neglected under typical drinking water treatment conditions [[17]]. References 1 [1]. P.C.Sharma, A. Jain and S. Jain, Fluoroquinolone antibacterial: a review on chemistry, Microbiology and therapeutic prospects, Acta Poloniae Pharmaceutica-Drug Res, Vol. 66 , 2009, pp. 587-604. [2]. P.Wang, Y.L. He and C.H. Huang, Oxidation of fluoroquinolone antibiotics and structurally related amines by chlorine dioxide: Reaction kinetics, product and pathway, Eval. Water res. vol.4 4, 2010, pp.5989-5998. [3]. M.C. Dodd , A.Shah ,U. V.Gunten and C. H.Huang, â€Å" Interactions of Fluoroquinolone Antibacterial Agents with Aqueous Chlorine: Reaction -Kinetics, Mechanisms, and Transformation Pathways† Environ. Sci. Technol. Vol.39, 2005, pp. 7065-7076. [4]. S. D. Richardson and T. A. Ternes, Emerging contaminants and current issues,† â€Å"Water analysis, Analytical Chemistry, vol. 77(12), 2005, pp. 3807–3838. [5] .J. Gibs, P. E. Stackelberg, E. T. Furlong, M. Meyer, S. D. Zaugg, and R. L. Lippincott, â€Å"Persistence of pharmaceuticals and other organic compounds in chlorinated drinking water as a function of time,† Science of the Total Environment, vol. 373(1), 2007 ,pp. 240–249. [6]. E.Rubinstein, History of quinolones and their side effects.† Chemotherapy 2001,47 (Suppl 3): 3 [7]. D.J. Diekema and R.N Jones. Oxazolidinone antibiotics. Lancet. 2001, 358 (9297):1975-82. Review. Pub Med PMID: 11747939. [8]. A.E. Barnhill, M.T. Brewer and S.A. Carlson. Adverse effects of antimicrobials via predictable or idiosyncratic inhibition of host mitochondrial components. Antimicrob. Agent. Chemother., 2012,Vol.56 (8):pp. 4046–4051. [9]. T C. Melton and S. D. Brown, Hindawi Publishing Corporation International Journal of Medicinal Chemistry, Vol. 2012, pp.1-6. [10] .S. H. Zinner, Antibiotic use: present and future, New microbiologica, Vol.30, 2007, pp. 321-325. [11] .H.C. Zhang, W. R. Chen and C.H. Huang Kinetic Modeling of Oxidation of Antibacterial Agents by Manganese Oxide, Environ. Sci. Tech., Vol. 42(15), 2008, pp. 5548–5554. [12]. S. D. Richardson and T. A. Ternes, â€Å"Water analysis: emerging contaminants and current issues,† Anal. Chem., Vol.77 (12), 2005, pp. 3807–3838. [13]. J. Gibs, P. E. Stackelberg, E. T. Furlong, M. Meyer, S. D. Zaugg, and R. L. Lippincott, â€Å"Persistence of pharmaceuticals and other organic compounds in chlorinated drinking water as a function of time,† Sci. Tot. Environ. Vol. 373(1), 2007, pp. 240–249. [14]. Z. Li, H. Fenet, E. Gomez, and S. Chiron, â€Å"Transformation of the antiepileptic drug oxcarbazepine upon different water disinfection processes,† Water Res., Vol. 45(4), 2011, pp. 1587– 1596. [15]. T.X. Wang and D.W.Margerum,. Kinetics of reversible chlorine hydrolysis: temperature dependence and general-acid/ base-assisted mechanisms. Inorg. Chem. Vol.33, 1994, pp.1050–1055. [16]. J.C.Morris, The acid ionization constant of HOCl from 5 to 351. J. Phys. Chem. Vol.70, 1966, pp.3798–3805. [17]. M. Deborde, U.V. Gunten, Reactions of chlorine with inorganic and organic compounds during water treatment—Kinetics and mechanisms: A critical review, water res., Vol. 42, 2008, pp.13 – 51.

Contrasting Shakespeares Richard with the Historical Figure Essay exam

There are two Richards: the Machiavellian monster created by Shakespeare and the historical figure who many historians claim is a much-maligned innocent man. So is Richard the sinner or the one sinned against? How can we decide? Is a decision even possible? In Shakespeare's play Richard III, Richard describes himself as a deformed malcontent in the opening soliloquy. (Shakespeare often uses physical deformity to mirror an evil mind.) I, that am curtail'd of this fair proportion, Cheated of feature by dissembling nature, Deformed, unfinish'd, sent before my time Into this breathing world, scarce half made up, ... And therefore, since I cannot prove a lover, To entertain these fair well-spoken days, I am determined to prove a villain And hate the idle pleasures of these days. (1.1.18-31) Many historians, on the other hand, have a different view of the man. For instance, in the 1956 biography Richard the Third, Paul Murray Kendall describes Richard based on contemporary writings and two well-known portraits of the King. Most contemporary descriptions bear out the evidence of these portraits that Richard had no noticeable bodily deformity, and establish him as a thin, frail man of a little less than normal height. (537) The most heinous crime that the Tudors (the kings who succeeded Richard to the throne) accused Richard of committing was the murder of his nephews-Edward V and Richard, Duke of York-the sons of his brother, the former king, Edward IV. How seriously should we take this accusation? What evidence supports it? Kendall writes, "If we take 'evidence' to mean testimony that would secure a verdict in a court of law, there is no evidence that he [Rich... ...ing skills that will lead to success both in school and in life. Works Cited Fields, Bertram. Royal Blood: Richard III and the Mystery of the Princes. New York: ReganBooks, 1998. Kendall, Paul Murray. Richard the Third. New York: Norton, 1983. - - - . Richard III: The Great Debate. New York: Norton, 1992. Looking for Richard. Dir. Al Pacino. Perf. Al Pacino, Alec Baldwin, Aidan Quinn, Winona Ryder, and Kevin Spacey. Videocassette. Fox, 1996. The Missing Princes of England. Dir. Melissa Jo Peltier. Narr. David Ackroyd. Videocassette. New Video Group, 1998. Shakespeare, William. Richard III. Ed. Barbara A. Mowat and Paul Werstine. New York: Washington Square Press, 1996. Tey, Josephine. The Daughter of Time. New York: Scribner, 1988. Weir, Alison. The Princes in the Tower. New York: Ballantine Books, 1995.

Animal Sciences Investigatory projects Essay

Anabas testudineus and its Preliminary Growth Adaptive Mechanism Through its Evolutinary Pathway To Brackish Water 4. Abstract: †¢ This study had mainly focused on the testing of the lifespan of a nearly endangered fish species Anabas testudineus in different water treatments namely brackish water, fresh water and salt water. The researchers determined the effect of the said water treatments on the growth and survival of the subjects. The study was conducted at the ESEP Bldg., Tarlac National High School (Main). The researchers applied the Completely Randomized Design (CRD) for the experiment. The length and mass of Anabas testudineus were recorded before and after the experimentation. The researchers prepared 3 containers for each replicate. Artificial environments were prepared in each container wherein 1000 grams of sand and pebbles and 400 grams of lilies were put in each container. The required amount of marine salt and water were also added and the pH of each water treatment were also obtained three randomly selected Anabas testudineus were subjected to each water treatment in each replicate. Everyday the stability of the fishes were observed. After 31 days, the final length and mass of the fishes were obtained and the results were compared. 5. †¢ The average difference in mass of the fishes subjected to brackish water is 2.945 grams higher than freshwater and the average difference in length is also higher than fresh water by 1.265 cm. †¢ Brackish water is the best water treatment for the growth of Anabas testudineus. †¢ Due to environmental changes evolution occurred as years passed by. The salinity of water and its pH are examples of these environmental changes. 6. The Efficacy of Moringa oleifera (Malunggay) Leaf extracts in Lowering Blood Glucose in Swiss Mice 7. †¢ Abstract: †¢ Malunggay (Moringa oleifera) plant is abundant through the Philippines. This plant is growing anywhere in the country. It has become the buzz due to the discovery of its many nutrients. The researcher tends to study on medical value of Moringa oleifera in lowering blood glucose. †¢ This study was performed to determine the effectiveness of Moringa oleifera leaf extracts in lowering blood glucose. The experimental mice where weighed and their blood glucose were tested using a glucometer before the experiment. †¢ The mice were fed with condensed milk using the gavage method for five (5) consecutive days. All the mice gained high blood glucose after the procedure. 8. †¢ Different concentrations of malunggay leaf extracts: 25%, 50%, 75% and 0% were prepared. These extracts were treated to three (3) mice each as treated to the four groups of mice. †¢ Data analysis revealed that there was a significant difference in the mean blood glucose level of white mice when subjected to various levels of malunggay leaves extract, the lower the blood glucose, †¢ The findings confirmed that the malunggay leaf extract has a hypoglycemic property that can be used as hypoglycemic drugs. Thus, it can be used in treating diabetes. 9. An extracorporeal Study on Nasturtium officinalle for Mus musculus Alleviating Thromboccytopenia 10. †¢ Abstract: †¢ The study was conducted at room 2, ESEP building, Tarlac National High School (Main). It started by sending every subjects blood sample to the laboratory to determine the initial platelet count of the subjects. Watercress undergoes centrifugation to attain its liquid content and was fed to a group of subjects. Observation lasted for three days then new blood sample was sent to the laboratory to determine the final platelet count of the subjects. Results were compared to prove that the researcher hypothesis is true.] †¢ The researcher used completely randomized design to compare the platelet count of the subject’s blood before and after was fed with the liquid content from watercress. The researcher used T-test because they are only two variables to compare. After series of test, the results were gathered. The data revealed that the mean platelet count from the finished product of the study is higher than the platelet count from the commercial anti- Thromboccytopen ia. 11. †¢ This study is mainly focus on the possibility of watercress in alleviating Thromboccytopenia, a disease characterized by low platelet count. To test this possibility, the researcher compared it to a commercial anti- Thromboccytopenia. The researcher determined the significant difference between the plasma produced from watercress and the plasma produced from a commercial anti- Thromboccytopenia(aspirin) in terms of platelet count. That is why the researcher must know if the platelet count can be produced faster by watercress. This study will contribute greatly to the world f medicine as an evidence to remedy diseases through natural, safer and cheaper means. It will be significant for the treatment of Thromboccytopenia without the used of chemicals and surgeries. 12. The Efficacy of Anacardic acid from Anacarduim occidentale as a Lipid Source of Oreochromis nil. 13. †¢ Abstract: †¢ Lipids are one of the molecules used by fishes for their physiological functions and cellular processes. These lipids have many uses in the lives of fishes, not only in fishes but also in other activities of other organisms. †¢ Oreochromis niloticus (tilapia) is one of the most common fish that contains lipids in their muscle or tissues. †¢ Anacadium ocindentale (cashew) is a nut crop with fruits that contain a liquid known as cashew nut shell liquid (CNSL). This three liquid has three phenol constituents, one of this is anacardic acid. 14. †¢ In this study, the cashew nut shell liquid (CNSL) was extracted from Anacardium occidentale and anacardic acid and then separated from the CNSL by the process of centrifugation and then mixed with the food of the fish and then fed to the fishes. The purpose of this study is to make a useful lipid source from anacardium occidentale. This study will test the efficacy of anacardic acid as a lipid source of Oreochromis niloticus, and observed if the growth development and weight increased. †¢ After observing the fishes for 1 month to 2 weeks, results and data were analyzed. The researcher used T-test as their statistical tool, and concluded that the growth and weight of the Oreochromis niloqticus increased by 50.36% in weight gain and 50.98% in the increase in size when given anacardic acid. 15. Residual Fatty Acids from Persia Americana for the Extension of Life Expectancy of Tirumala tumanana 16. †¢ Abstract: †¢ The study had been centered on the efficacy of amalgamated fatty acid residues from Persia Americana, the fatty acids were extracted at the Philippine Institute of Pure and Applied Chemistry (PIPAC) using the gc mini2 machine for the procedure of gas chromatography. †¢ This study further branched out into the possible effects of the fatty acid residues in extending the life expectancy of Tirumala tumanana, specie of butterfly originated at Mt. Busa and is rated as vulnerable in the International Union for Conservation of Nature (IUCN). 17. †¢ Three treatments were designated to 24 butterflies: Treatment A (with flowering plants, sugar solution, and fatty acids), treatment B (with flowering plants and sugar solution), and Treatment C (with flowering plants only). †¢ ANOVA (Analysis of Variance) was utilized to compare the three treatments in observing the life expectancy of Tirumala tumanana. †¢ After the series of test and experimentation, the results showed that the average life span of the subjects was prolonged by 129.03%, enough to say that there is a significant effect produced by Persea Americana. 18. Behavioral and Social Science 19. A Study on the Effect of Food Outsourcing to the Behavioral Direction of Trichogaster Pectoralis and Tricogaster Tricopterus 20. †¢ Abstract: †¢ Trichogaster Pectoralis and Tricogaster Tricopterus- these two species have the same denominator; they belong in one genus- trichogaster. Since they came from one genus, they have mostly the same characteristics like what kind of environment they adapt, what food they eat, and their behavior. †¢ This study aims to establish the effect of food outsourcing on the direction decision of Trichogaster Pectoralis and Tricogaster Tricopterus subjects based on attraction and non-attraction. It also aims to give additional knowledge to fish cultivators, and other persons involved in aquaculture. 21. †¢ The set-up where will the experimentation be concluded is composed on an aquarium, aquarium air pump, 15L of fresh water and 8 subjects which were drawn out randomly but on accordance with the ratio stated on each of the two treatments. The animal subjects underwent the process of food outsourcing where 20g of fish food is used, observation and data gathering for 10 days. The data gathered were then compared, and unbiased and reliable results were obtained. Results showed that food outsourcing affected the behavioral direction of the subjects based on attraction. Thus, the null hypothesis was rejected, accepting the alternative hypothesis which states that the effect of food outsourcing on the Behavioral Direction of Trichogaster Pectoralis and Tricogaster Tricopterus based on attraction is greater than non- attraction. †¢ In line with the results and the accepted hypothesis, the researchers concluded that the effect of food outsourcing on the behavioral direction of the Trichogaster Pectoralis and Tricogaster Tricopterus subjects is to be attracted from one another. †¢ 22. Plant Science 23. Zeolite (Clinoptilolite) as a soil nutrient Enhanser 24. †¢ Abstract: †¢ This research study entitled â€Å"Zeolite (Clinoptilolite) as a soil Nutrient Enhancer† was conducted at Abing’s Residence located at Mabini Homesite Cabanatuan City. It was conducted from the month of June to October 2008 by the following researcher; Jessamer D. Abing. †¢ Because of the economic crisis that our country is facing today, most of the less fortunate people and farmers in our country cannot afford such expensive fertilizer used in growing plants. The goal of this study is to find an alternative soil nutrient enhancer out of the abundance of Zeolite mineral that can be used in growing plants in easier and mire practical way. 25. †¢ To test the effectiveness of Zeolite in growing plants, 3 treatments were utilized: Each treatment is composed of three plants. Each specimen has its own treatment, treatment 1 is a mixture of oil and powdered Zeolite (75g), treatment 2 is a mixture of soil and commercial fertilizer (75g) a nd treatment 3 is the plane soil. †¢ The results was analyzed and found out that in terms of mineral content in Phosphorus and Nitrogen there is a significant difference between soil with Zeolite, soil with commercial fertilizer and plain soil and in terms of Potassium Contents there a significant difference among 3 samples because Zeolite yields the greatest amount of Potassium. In terms in the growth of the plants, Zeolite was the most effective soil nutrient enhancer because the plants with Zeolite yield the tallest and healthiest appearance among the 3 specimens. †¢ This proves that Zeolite is very effective and efficient soil nutrient enhancer which can be use by the farmers in growing plants. 26. Gralex (Ready-Made Bait for Mice) 27. †¢ Abstract: †¢ The utilization of â€Å"Gralex† as ready made bait for mice, aimed to test its toxicity on mice samples. †¢ Systematic methods in the preparation of Gralex were observed. Three (3) treatments which varies on the soaking time of rice grains and adelfa leaves extracts such as forty- eight (48) hours, twenty-four (24) hours, fifteen hours (15) respectively. †¢ Prior to experimentation, Adelfa leaves underwent phytochemical testing. Result revealed the presence of condensed tannin, alkaloids and glycosides compound. †¢ Toxicity level of Gralex is higher as compared to commercial rat killer (Racumin) as observed on the mortality rate of mice. †¢ Statistical test result showed significant difference on the effectiveness on the rat killers. Gralex was found to be more effective. It was concluded that the presence of Tannins and Glycosides contributed a lot in mortality rate on the mice samples. †¢ The production and commercialization of the product is strongly encourage. 28. Tolerance Level of Meristematic Root Cells of Bruguiera gymnorrhiza to High and Low Hypertonic Saline Solutions and Hypotonis Solutions 29. †¢ Abstract: †¢ Plants extinction is already a global major problem but ordinary people are not aware of this. The worst part is that is very important and gives us the most benefits are the one that are disappearing. One good example of this plant is mangrove tree. †¢ These mangroves give us a lot of benefits. For fishes, these provide breeding, nursery, and feeding areas. These can tolerate more salt in their tissues than any plant. Their survivability is high compared to other plants but they also need regular flushing of freshwater. 30. †¢ The problem of this study is to determine the tolerance level of the Bruguiera gymnorrhiza meristematic root cells to high and low saline hypertonic solution and hypertonic solution. The proponents conducted the study at ESEP, DOST building, TNHS, San Roque Tarlac City. The experimentation started at Feb. 27, 2010 and ended last Aug.22, 2010. The proponents prepared 4 containers containing water with different salinities (0ppm, 30ppm-35ppm, 36ppm-45ppm, 46ppm- 50ppm). Three roots of the mangrove per solution were soaked and we let it that way for few hours. After soaking, a part of the mangrove roots were cut and observed under a compound microscope. Cells that shrink and burst were counted and the tolerable salinity of the Bruguiera gymnorrhiza root cells was known. 31. Natural Extracts as Vase Solution for Roses (Rosa damascena) 32. †¢ Abstract: †¢ This study was conducted to determine the effects of plant extracts from garlic allium sativum, Oregano Origanum vulgare, and guava Psidium guajave) as vase solution. Extract used for the preparation of vase solutions were taken from fresh garlic cloves, oregano and guava leaves. Water extracts were subjected to water bath at 50’C-60’C for 5 minutes for pasteurization. Flower buds were placed in plastic cups containing vase solutions with varying concentrations of extracts. Observation was done for 7 days at three replications. Set-ups were evaluated in terms of pH. Early opening of flowers and length of Vaseline days. †¢ Analysis on pH differences was not significant among treatments. Oregano and guava leaves may have favorable pH levels for flowers used. In terms of opening of flowers, it was noted that oregano extract promoted early opening of flower buds even at the early days of observation. Highest vase life days were also observed in flowers placed in guava leaves extract and the lowest vase life days were observed in flowers placed garlic extract. 33. †¢ Analysis of variance (ANOVA) at 0.5 level of significance did not reveal differences in the vase life days of flowers after treatment with natural extracts from Garlic, Oregano and Guava. A modification of procedures for extracting natural compounds from guava is recommended. The researchers also recommended the combinations of extract for more favorable results. This research has varied application in the field of Horticulture and food production. 34. Larvicidal Properties of Various Plant Species in the Control of Mosquito Larvae 35. †¢ This study was conducted to identify several plant species that contain †¢ Larvicidal properties in the control of mosquito larvae and to determine the time needed to kill the mosquito larvae. A total of 240 larvae were used to test the larvicidal properties of the treatment. The test plants were subjected to certain procedures like through washing, chopping, and pounding to extract the plant juice concentrates. A cheesecloth was also used in extracting the juice after pounding through the used of mortar and pestle. The mosquito larvae were then placed in their respective treatments. They were observed based on the time interval of two, four, and six hours. The Analysis of Variance (ANOVA) of the Completely Randomized Design (CRD) was used to analyze the data collected. 36. †¢ The treatments used were as follows: †¢ T0- Water (control) †¢ T1- Neem tree †¢ T2- Kakawate †¢ T3- Makabuhay †¢ T4- Karot †¢ T5- Guava †¢ T6- Patani †¢ T7- Chili Pepper †¢ Results of the study showed that among the eight treatments, T2-(Kakawate) had the best result in controlling mosquito larvae. Based on the results of the study, water has no component that could kill mosquito larvae. †¢ This study was a big help to control mosquito population that can be diluted to potable water to kill the mosquito larvae. 37. Zoology 38. Ar-aru: A small fish and its Backward Evolution 39. †¢ Abstract: †¢ More than 3.5 billions years ago, the first organisms is believed to have live in water namely as the organisms cyanobacteria, oxygen was not present which then gives the parameter that these organisms are anaerobic and as years passed by, changes occur. These changes are called evolution. Salinity is one of these changes, Sodium Chloride came fro rocks in freshwater bodies are washed out and the water changing this NaCl will be deposited in saltwater bodies. †¢ Because of this, the average salinity of salt water is 17ppt. Freshwater having the salinity between 0.5 ppt. to 17ppt. is called brackish water and can be found in the boundary of saltwater and freshwater. More than half of the fauna in the Philippines face the treat of extinction. A fish called Ar-aru is now facing this treat. †¢ This project mainly studies the effects of water salinities on the growth of Ar-aru fishes in terms of mass, length, mortality and reproductive mechanism. 40. †¢ To make the study possible, the researcher had undergone series of experimentation and observation of their adaptability on treatment F (Freshwater), treatment B (Brackish) and treatment S (Saltwater) with 2 replicates each. The data gathered showed that Ar-aru fishes subjected to brackish water showed positive result on their adaptive mechanism in terms of its survivability. Thus, the salinity of water is a factor that affects the survivability of this specie. †¢ Results showed that raising ar-aru fishes in brackish water is better than in freshwater and saltwater. This implies that the ar-aru fishes had an adaptive mechanism to cope in the environment of brackish.

Indian Removal Act Essay

The Indian Removal Act of 1830 was a heated topic in Congress. Defend the passage or rejection of the Act with reference to the moral, political, constitutional and practical concerns of a congressman. The Indian Removal Act of 1830 was at the time, thought to be justified and acceptable. There were two groups, the people who wanted the Indian’s gone, and the people who believed they should be allowed to stay. I believe forcing the Indians out of their territory was immoral, had no effect on the state of Georgia, and it was an abuse of power. When the Indians were being removed from their territory, they were lead out at gunpoint by our military. It’s immoral because there were 17,000 Indians and 4,000 died due to dehydration, starvation, and disease. The Cherokee had rights given to them. â€Å"They were granted their separate existence, as a political community, undisturbed possession and full enjoyment of their lands, within certain boundaries, which are duly defined and fully described and the protection of the United States against all in interference with, or encroachments upon, their rights, by any people, State, or nation,† argues a Maine Senator. The Indian Removal Act disregards every set of promises we have given to them. â€Å"What is the population of Georgia, where there is no room for these few Indians? It is less than seven to the square mile. We, Sir, in Massachusetts, have seventy-four to the square mile, and space for a great many more,† states a Congressman. The occupation of Indians in Georgia had little effect on the society or development of it. Georgia wanted to â€Å"consolidate their society† but they weren’t going to strengthen their community by kicking people out. President Andrew Jackson and President James Monroe, both were for the Indian Removal Act. President Jackson asks, â€Å"What good man would prefer a country covered with forests and ranged by a few thousand savages to our extensive republic?† The Constitution of 1789 gave Congress the power to regulate commerce with foreign nations, and among the several States, and with the Indian tribes. Yes, this is part of the Constitution, but the abuse of power comes in here. We force them out of their territory that we occupied, with guns to their heads, no food, and the separation of their families. I have always surmised that the Indian Removal Act was inhumane, unfair, and ill advised, but I never really knew why I thought that. After reading about it, looking at both sides, it’s clearer. The Indian’s were forced out for a reason that had no effect on the states, and it was abusing the power that the United States had been working for.

Paper Making Industry

Paper Making Industry Introduction Today, there is massive competition in the corporate world. Companies continually seek ways to stay in business and to outdo their rivals. Consequently, many institutions have embraced advertising to stay in business. There are many ways to advertise especially by use of media. In this century, use of internet and audios include students, teachers, parents, employers, and employees among other people. The widespread use of the paper shows its importance. People mainly use papers to communicate. However, papers have advanced uses such as making decorations and bags. Used papers are also useful. People recycle them to make more tissue papers, cartons, and decorations among other uses.Advertising Looking for essay on business economics? Let's see if we can help you! Get your first paper with 15% OFF Learn More It is essential for individuals to use papers efficiently. This is because the resources used come from the environment, which is susceptible to degradation and pollution that results from the paper making processes. People should maintain papers by using them for the right purposes. In addition, individuals should put used papers to proper use. This way the papermaking life cycle will be less harmful to the environment (European Commission 1). Waste management Evidently, papers are essential to a large population in running their everyday duties. This makes industries related to paper and pulp industry important as they play an integral role in the social and economic developments. However, these industries pose challenges to the environment. In their bid to satisfy the overwhelming demand for papers, Pulp and paper industries emit large amounts of waste throughout the production processes. These wastes range from primary sludge, organic wastes, to greenhouse gases. Factories relea se emissions and wastes in all stages of paper production. In the first stage, waste consist of rejected virgin pulps, sand, remains of woods, and barks, which the factory cannot use in subsequent processes. During the chemical recovery process, the factory emits wastes such as green liquor sludge, lime mud, wastewater, and chemical waste sludge. When making paper the mills emit wastes such as fibers, wool, and impurities like metal and staples. Further, paper and pulp industries produce harmful air emissions. Components such as electricity-generating units produce gases such as sulfur oxide, nitrogen oxide, methane, and volatile organic compounds. On top of being malodorous, these emissions cause massive air pollution (Venditti 17). Stakeholders of the paper and pulp industry have a responsibility towards management of wastes and emissions related to this industry. The government on its part should enforce firm regulations. It should also spell out the limits of this industry. The public should ensure that this industry does not compromise their safety through pollution of the environment. These factories should recover wastes and emissions, and convert them into useful products. For example, the factories should use greenhouse gases to generate heat and energy in the manufacturing plants (Office of Air and Radiation 12). In addition, these factories should minimize the generation of wastes. They should embrace installations that save on energy. Further, they should embrace new and convenient techniques. In case of closure, plants should leave the location in its original state or in an improved state. Further, these factories should engage in waste recovery processes such as wet oxidation and steam reformation. The factories should ensure safe and convenient disposal of their emissions and wastes. The factories should also engage in recycling of wastes. For example, factories can use barks and rejected pulps as fuel for the furnaces. These factories can use treated wastewater in the production processes. Finally, the factories should reclaim land and engage in activities that enhance soil fertility. Summary of research findings When conducting research, an individual gets to learn a lot and encounters several challenges. The factory management in many firms is ready to talk of the achievements of their companies. However, it is hesitant to open up on the challenges and flaws of the firms. This investigation was a success in terms of information gained. The information gathered on the general procedure of paper manufacturing process was efficient. Today, the paper industry is one of the big industries in the world. Many people use the paper widely in their everyday activities. This has led to the growth of this industry. There is a booming market for paper products in countries like India and the US. This has contributed to the massive production of the papers. This industry has two main sub-sectors. The first sub-sector deals with manu facture of pulp and paper while the latter concentrates on manufacturing paper products. Manufacture of paper is the core activity of this industry. Most factories in the industry engage in the manufacture of both paper and converted paper products. This industry has witnessed a series of transformations when it started (U.S Censors Bureau 1). Technological advancement and extensive research have propelled the industry forward and helped to improve its quality. As the investigation established, the stakeholders have put more effort in order to improve this industry. However, this industry faces major challenges. Being a universal industry, it requires many resources for it to function. Most of these resources are renewable while several are non-renewable. Further, there are large numbers of harmful wastes and emissions that result from the production processes. The investigation carried out failed to establish permanent and lasting solutions to some of the problems faced by this ind ustry. No factory had a lasting solution regarding safe disposal of waste and re-use of harmful wastes. It was a challenge for the research to find out the truth regarding the actual processes involved in disposal of wastes and emissions in these factories. The research had to make assumptions for it to move forward with the investigations. Such assumptions rotated around the technicality involved in the entire production process. For instance, the research assumed that the life expectancy of this industry would be shorter if the industry does not take precautionary measures. The research evaluated the impact of the increased use of internet instead of papers. Further, the research assessed the consequences of the environmental issues to the industry. Conclusion Importance of paper making industry in the newspaper advertisement process is evident. It is essential to understand the life cycle of the paper-manufacturing sub-sector. The life cycle assessment estimates the amount of mat erials and energy that factories consume in the process of producing papers. Further, the life cycle assessment calculates the amount of wastes and emissions released to the environment by the factories. Overall, the life cycle assessment method aims at establishing the impact of the production processes to the environment. Researching on this process enables individuals to understand the different sub-sectors involved in production of a single product. For instance, designing of a newspaper depends on sub-sectors such as paper, ink, electricity, and transport industries. Therefore, all these subsectors should consider the impacts of their functions on the environment and strive to maintain ecological balance for a sustainable ecosystem (Krowaski, Bresky and Pettersson11 ). Carnegie Mellon University. EIO-LCA: Free, Fast, Easy Life Cycle Assessment. n.d. Web. European Commission. Joint Research Center. 2013. Web. Krowaski, klaus, Jan Bresky and BÃ ¶rje Pettersson. A Life Cycle Ass esment of the Production of a daily Newspaper and Weekly Magazine. Zurich: Axel Spirnger Verlag AG , 1998. Print. Lecta Group. About Paper Manufacturing. 2008. Web. Office of Air and Radiation. Available and Emerging Technologies for Reducing Greenhouse Gases emission from the Pulp and Paper Manufacturing Industry. 2010. Web. Roekel, Gertjan. Hemp Pulp and Paper Production. 1994. Web. U.S Censors Bureau. NAICS 322: Paper Manufacturing. n.d. Web. Venditti, Richard. Life Cycle Analysis of Paper Products. 2011. Web.

An Overview of Territories of the World

An Overview of Territories of the World While there are fewer than two hundred independent countries in the world, there are more than sixty additional territories that are under the control of another independent country. There are several definitions of territory but for our purposes, we are concerned with the most common definition, presented above. Some countries consider certain internal divisions to be territories (such as Canadas three territories of Northwest Territories, Nunavut, and Yukon Territory or Australias Australian Capital Territory and Northern Territory). Likewise, while Washington D.C. is not a state and effectively a territory, it is not an external territory and thus not counted as such. Another definition of territory usually is found in conjunction with the word disputed or occupied. Disputed territories and occupied territories refer to places where the jurisdiction of the place (which country owns the land) is not clear. The criteria for a place being considered a territory are fairly simple, especially when compared to those of an independent country. A territory is simply a external piece of land claimed to be a subordinate location (in regards to the main country) that is not claimed by another country. If there is another claim, then the territory can be considered a disputed territory. A territory will typically rely on its mother country for defense, police protection, courts, social services, economic controls and support, migration and import/export controls, and other features of an independent country. With fourteen territories, the United States has more territories than any other country. The territories of the U.S. include: American Samoa, Baker Island, Guam, Howland Island, Jarvis Island, Johnston Atoll, Kingman Reef, Midway Islands, Navassa Island, Northern Mariana Islands, Palmyra Atoll, Puerto Rico, U.S. Virgin Islands, and Wake Island. The United Kingdom has twelve territories under its auspices. The United States Department of State provides a nice listing of more than sixty territories along with the country that controls the territory.

5 Study Secrets to Ace Your Exams

5 Study Secrets to Ace Your Exams Most students hate tests. They hate the feeling of trying to remember the answer to a question, worrying that they focused on the wrong material, and waiting to receive their results. Whether you learn at a traditional school or study from the comfort of your own home, chances are you’ll have to sit through many test-taking experiences. But there are a few tricks you can learn now to avoid the worry before you’re in the heat of the moment. Give these five proven study tips a try and see how much better you feel during your next exam. 1. Survey your textbook or workbook before you read. Take a couple of minutes to find the glossary, index, study questions and other important information. Then, when you sit down to study, you’ll know where to find the answers you are looking for. Make sure you read any study questions before you read the chapter. These questions let you know what you can probably expect in any upcoming tests, papers or projects. 2. Attack your textbook with sticky notes. As you read, summarize (write down the main points in just a few sentences) each section of the chapter on a post-it note. After you have read the entire chapter and summarized each section, go back and review the post-it notes. Reading the post-it notes is an easy and efficient way to review information and, because each note is already in the section it summarizes, you can easily find the information you need. 3. Use a graphic organizer to take notes when you read. A graphic organizer is a form you can use to organize information. As you read, fill out the form with important information. Then, use your graphic organizer to help you study for the test. Try using the Cornell notes worksheet. Not only does this organizer let you record important terms, ideas, notes and summaries, it also lets you quiz yourself on that information by folding the answers upside down. 4. Make your own practice test. After you finish reading, pretend you are a professor who is writing a test for the chapter. Review the material you just read and make up your own practice test. Include all vocabulary words, study questions (they’re usually at the beginning or end of the chapter), and highlighted words you can find, as well as any other information you think is important. Take the test you’ve created to see if you remember the information. If not, go back and study some more. 5. Create visual flashcards. Flashcards aren’t just for primary students. Many college students find them useful as well. Before you take a test, make flashcards that will help you remember important terms, people, places and dates. Use one 3-by-5-inch index for each term. On the front of the card, write down the term or question you need to answer and draw a picture that will help you remember it. This will help ensure that you grasp the study material as you’ll find that it’s almost impossible to sketch something you don’t really understand. On the back of the card write down the definition of the term or the answer to the question. Review these cards and quiz yourself before the actual test.